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high performance liquid chromatography hplc analysis  (Hitachi Ltd)


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    Hitachi Ltd high performance liquid chromatography hplc analysis
    High Performance Liquid Chromatography Hplc Analysis, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 99/100, based on 2000 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hplc+analysis/Chromaster/10__1016_slash_j__fochx__2026__103898-110-17-23
    Average 99 stars, based on 2000 article reviews
    high performance liquid chromatography hplc analysis - by Bioz Stars, 2026-09
    99/100 stars

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    Derivatization:

    Article Title: Multilayered substructures of a non-enzymatic chemical reaction network synthesizing sugars
    Article Snippet: .. The 15 derivatization was performed by adding 748 μL of water, 375 μL of DNPH solution in acetonitrile (1 mg mL−1), 16 and 22.5 μL of 20 vol% aqueous phosphoric acid to 2.5 μL of a sample solution followed by stirring at room 17 temperature for 1 h. HPLC analysis was performed using a Chromaster® (Hitachi) equipped with a diode array 18 detector (360 nm; 5430 diode array detector, Hitachi) and 5110 pump (Hitachi). .. InertSustain C18 column (5 19 μm, 4.6 × 150 mm, GL Sciences) was connected and mixed solution of water-acetonitrile (6:4, v/v) was used 20 as the mobile phase at a flow rate of 1.0 mL min−1.

    High Performance Liquid Chromatography:

    Article Title: Multilayered substructures of a non-enzymatic chemical reaction network synthesizing sugars
    Article Snippet: .. The 15 derivatization was performed by adding 748 μL of water, 375 μL of DNPH solution in acetonitrile (1 mg mL−1), 16 and 22.5 μL of 20 vol% aqueous phosphoric acid to 2.5 μL of a sample solution followed by stirring at room 17 temperature for 1 h. HPLC analysis was performed using a Chromaster® (Hitachi) equipped with a diode array 18 detector (360 nm; 5430 diode array detector, Hitachi) and 5110 pump (Hitachi). .. InertSustain C18 column (5 19 μm, 4.6 × 150 mm, GL Sciences) was connected and mixed solution of water-acetonitrile (6:4, v/v) was used 20 as the mobile phase at a flow rate of 1.0 mL min−1.

    Article Title: Metabolic activation of pyruvate cycle by protocatechualdehyde triggers oxidative killing of ampicillin-resistant Escherichia coli
    Article Snippet: Every 1 mL of supernatant was transferred to a 1.5 mL QSP centrifuge tube, and the supernatant was enriched with a balanced concentrator (Model: EYELA/CVE-3000) at a pressure of 24 hPa and a rotational speed of 6. .. The completely dried samples were reconstituted with 40 μL of methanol and transferred to the liner tube of a sample vial for HPLC analysis (model: Chromaster/Primade; manufacturer: Hitachi, Japan). ..

    Article Title: Optimizing transplanting and harvesting dates for ‘Hopungmi’ sweet potato (Ipomoea batatas L.) in Yeoju under climate change
    Article Snippet: Sweet potato is mainly consumed as a staple food in developing countries due to its high energy output compared to most major food crops.. Recently, it has also been recognized as a functional food due to its high content of phytonutrients, such as anthocyanins, carotenoids, and phenolic compounds, which provide balanced nutritional and antioxidant properties (Katayama et al. 2017; Xiao et al. 2022).. In addition, sweet potato is used for various purposes, such as animal feed, brewing raw materials, and bioethanol (Honma and Yamakawa 2019).

    Article Title: Chiral 8-Amino-5,6,7,8-tetrahydroquinoline Derivatives in Metal Catalysts for the Asymmetric Transfer Hydrogenation of 1-Aryl Substituted-3,4-dihydroisoquinolines as Alkaloids Precursors
    Article Snippet: .. Catalytic reactions were monitored by HPLC analysis using Merck-Hitachi L-7100 (Merck-Hitachi, Darmstadt, Germany) equipped with Detector UV6000LP and chiral column (Chiralcel OD-H, Chiralpak AD, Lux Cellulose-2 or Lux Amylose-2). .. Novozym 435 from Sigma-Aldrich (immobilized on acrylic resin, ≥5000 U/g, recombinant, expressed in Aspergillus niger ).

    Article Title: Method for production of sterile flowering biomass in temporary immersion bioreactors
    Article Snippet: .. HPLC analysis: HPLC analysis were conducted on a Chromaster (Hitachi) 600 bars system, with a gradient pump 5160, sampler 5260, column oven 5310, pump with degasser 5160, diode array detector DAD 5430, Chromaster software, connected to a Dell Optiplex 3040 PC. .. Separation was done using a Kinetex® Core Shell C18 column (Phenomenex, 2.6 μm, 100A, 150×3 mm), protected by a precolumn (Phenomenex, SecurityGuardTM ULTRA Cartridges, UHPLC C18 3.0 mm ID Columns, mounted on SecurityGuardTM ULTRA Holder for UHPLC Columns 2.1 to 4.6 mm ID).

    Article Title: Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine
    Article Snippet: Amino acids were quantified by HPLC (Agilent 1200 Series, SIM, Oberhausen, Germany) involving pre-column derivatization and fluorescence detection as described previously [ ]. .. Quantification of ectoine and hydroxyectoine was performed by HPLC analysis (LaChrome, Merck-Hitachi, Darmstadt, Germany) using a ProntoSil C18 AQ + column (125 × 4 mm, Knauer, Berlin, Germany) with a Nucleosil C18 AQ + pre-column (120 × 5 mm, Knauer, Berlin, Germany). ..

    Article Title: Phenylpropanoid volatile synthesis during sequential roasting of Lu’an Guapian green tea: Differential activities of gallocatechin isomers in aromatic amino acid pyrolysis and Maillard reactions
    Article Snippet: .. The filtrate was diluted 5 folds with 70% ( v /v) methanol before HPLC analysis (Hitachi Primaide, Japan). .. 10 μL of sample was injected into LaChrom C 18 column (4.6 mm × 250 mm, 5 μm particle size, Hitachi, Japan) which was maintained at 36 °C.

    Software:

    Article Title: Method for production of sterile flowering biomass in temporary immersion bioreactors
    Article Snippet: .. HPLC analysis: HPLC analysis were conducted on a Chromaster (Hitachi) 600 bars system, with a gradient pump 5160, sampler 5260, column oven 5310, pump with degasser 5160, diode array detector DAD 5430, Chromaster software, connected to a Dell Optiplex 3040 PC. .. Separation was done using a Kinetex® Core Shell C18 column (Phenomenex, 2.6 μm, 100A, 150×3 mm), protected by a precolumn (Phenomenex, SecurityGuardTM ULTRA Cartridges, UHPLC C18 3.0 mm ID Columns, mounted on SecurityGuardTM ULTRA Holder for UHPLC Columns 2.1 to 4.6 mm ID).

    Article Title: Flavonoids extract from Rosa roxburghii fruit ameliorates carrageenan-induced thrombosis in mice
    Article Snippet: Metabolic profiling was performed using a Sciex QTrap 6500+ (SCIEX, Redwood City, CA, USA) with ionization parameters: Ion-Spray Voltage: +5500/− 4500 V, DP: ±100 V, Curtain Gas: 35 psi, Ion Source Gas 1:60 psi, Ion Source Gas 2: 60 psi. .. HPLC analysis was performed on a Primaide system (Hitachi, Tokyo, Japan), which consists of an LC with UVD and is controlled by a ChromAssist Data Station software workstation. .. AQ-C18 (4.6 mm × 250 mm, 5 μm) (GL Science, Tokyo, Japan) was used as the column.



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    Body coloration phenotypes and carotenoid contents following RNAi-mediated gene silencing in Tetranychus urticae females reared under diapause-inducing conditions. (A) Representative images of the three body color phenotypes scored after dsRNA treatment: orange (typical diapause phenotype with high carotenoid accumulation), yellow (intermediate, altered carotenoid composition), and green (reduced diapause-associated carotenoid accumulation). Scale bars, 0.1 mm. The stacked bar chart represents the proportion of individuals classified as orange, yellow, or green within each treatment group following dsRNA treatments targeting the TuPDS ( tetur01g11270 ), TuLCPS ( tetur01g11260 ), TuCYP384A1 ( tetur38g00650 ), or TuPLAT10 ( tetur11g05720 ) genes (ds TuPDS , ds TuLCPS , ds TuCYP384A1 , or ds TuPLAT10 , respectively), compared with the dsRNA treatment targeting an intergenic region used as a negative control (dsNC). Different letters above the bars indicate significant differences among treatments (Fisher’s exact test, p < 0.001); n , total number of mites scored. Of females classified by body coloration phenotype, the body-color subgroups containing sufficient individuals were subjected to high-performance liquid chromatography <t>(HPLC)</t> analysis: yellow only for ds TuLCPS ; yellow and green for ds TuPDS , ds TuCYP384A1 , and ds TuPLAT10 . dsNC females (all orange) and non-diapausing females (all green) were each analyzed as a single group. HPLC analysis for quantifying the contents of (B) β-carotene and (C, D) astaxanthin in dsRNA-treated mites and non-diapausing mites. (C) Cholesterol esterase treatment (+CE) hydrolyses esterified astaxanthin into its free forms. In (D), −CE indicates samples without esterase treatment (free astaxanthin only). Data are mean ± standard error of the mean (SEM), with individual dots indicating biological replicates. Statistical significance relative to dsNC was determined using Dunnett’s test ( p < 0.001).
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    Image Search Results


    Body coloration phenotypes and carotenoid contents following RNAi-mediated gene silencing in Tetranychus urticae females reared under diapause-inducing conditions. (A) Representative images of the three body color phenotypes scored after dsRNA treatment: orange (typical diapause phenotype with high carotenoid accumulation), yellow (intermediate, altered carotenoid composition), and green (reduced diapause-associated carotenoid accumulation). Scale bars, 0.1 mm. The stacked bar chart represents the proportion of individuals classified as orange, yellow, or green within each treatment group following dsRNA treatments targeting the TuPDS ( tetur01g11270 ), TuLCPS ( tetur01g11260 ), TuCYP384A1 ( tetur38g00650 ), or TuPLAT10 ( tetur11g05720 ) genes (ds TuPDS , ds TuLCPS , ds TuCYP384A1 , or ds TuPLAT10 , respectively), compared with the dsRNA treatment targeting an intergenic region used as a negative control (dsNC). Different letters above the bars indicate significant differences among treatments (Fisher’s exact test, p < 0.001); n , total number of mites scored. Of females classified by body coloration phenotype, the body-color subgroups containing sufficient individuals were subjected to high-performance liquid chromatography (HPLC) analysis: yellow only for ds TuLCPS ; yellow and green for ds TuPDS , ds TuCYP384A1 , and ds TuPLAT10 . dsNC females (all orange) and non-diapausing females (all green) were each analyzed as a single group. HPLC analysis for quantifying the contents of (B) β-carotene and (C, D) astaxanthin in dsRNA-treated mites and non-diapausing mites. (C) Cholesterol esterase treatment (+CE) hydrolyses esterified astaxanthin into its free forms. In (D), −CE indicates samples without esterase treatment (free astaxanthin only). Data are mean ± standard error of the mean (SEM), with individual dots indicating biological replicates. Statistical significance relative to dsNC was determined using Dunnett’s test ( p < 0.001).

    Journal: bioRxiv

    Article Title: A single PLAT domain protein couples reproductive arrest and carotenoid pigmentation during diapause in the two-spotted spider mite, Tetranychus urticae Koch

    doi: 10.64898/2026.05.13.724795

    Figure Lengend Snippet: Body coloration phenotypes and carotenoid contents following RNAi-mediated gene silencing in Tetranychus urticae females reared under diapause-inducing conditions. (A) Representative images of the three body color phenotypes scored after dsRNA treatment: orange (typical diapause phenotype with high carotenoid accumulation), yellow (intermediate, altered carotenoid composition), and green (reduced diapause-associated carotenoid accumulation). Scale bars, 0.1 mm. The stacked bar chart represents the proportion of individuals classified as orange, yellow, or green within each treatment group following dsRNA treatments targeting the TuPDS ( tetur01g11270 ), TuLCPS ( tetur01g11260 ), TuCYP384A1 ( tetur38g00650 ), or TuPLAT10 ( tetur11g05720 ) genes (ds TuPDS , ds TuLCPS , ds TuCYP384A1 , or ds TuPLAT10 , respectively), compared with the dsRNA treatment targeting an intergenic region used as a negative control (dsNC). Different letters above the bars indicate significant differences among treatments (Fisher’s exact test, p < 0.001); n , total number of mites scored. Of females classified by body coloration phenotype, the body-color subgroups containing sufficient individuals were subjected to high-performance liquid chromatography (HPLC) analysis: yellow only for ds TuLCPS ; yellow and green for ds TuPDS , ds TuCYP384A1 , and ds TuPLAT10 . dsNC females (all orange) and non-diapausing females (all green) were each analyzed as a single group. HPLC analysis for quantifying the contents of (B) β-carotene and (C, D) astaxanthin in dsRNA-treated mites and non-diapausing mites. (C) Cholesterol esterase treatment (+CE) hydrolyses esterified astaxanthin into its free forms. In (D), −CE indicates samples without esterase treatment (free astaxanthin only). Data are mean ± standard error of the mean (SEM), with individual dots indicating biological replicates. Statistical significance relative to dsNC was determined using Dunnett’s test ( p < 0.001).

    Article Snippet: For HPLC analysis, standard β-carotene (Nacalai Tesque, Inc., Kyoto, Japan) and astaxanthin (Dr. Ehrenstorfer GmbH, Augsburg, Germany) were dissolved in chloroform to prepare stock solutions of each standard at 10 mg mL −1 .

    Techniques: Negative Control, High Performance Liquid Chromatography